Biochemistry lab I. Introduction In this experiment, lipids from ground nutmeg tree ar extracted using a combination of firmness of purposes and identify the lipids through chromatography. The use of goods and services of using solvent combinations is to elute the lipids base on their mutual opposition to binding of the silica jelly. The chromatography is performed on a silica change plate and the use of unity to visualize the lipids. By scheming the Rf values for individually compound and comparing them to the know lipids, we are able to diagnose the lipids within the grounded nutmeg. II.
Procedures Add 5 g crushed nutmeg and 50 mL hexane-isopropanol into a flaskful and warm for 15 minutes. Remove the extra solvent on a travel bath under a hood while flushing the flask with N2 gas, leaving the crude extract. consult extract. Prepare silica gel newspaper column. Add 6 g of silica gel in 20 mL of hexane to read a slurry. Block column with small piece of deoxyephedrine wool, add 5 mL of hexane and then add the silica slurry up t...If you want to outsmart a full essay, show it on our website:
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